METHODS OF MICROPROPAGATION:

Many ornamental plants and woody species are propagated commercially by shoot tip and axillary bud proliferation. Axillary bud proliferation typically results in an average tenfold increase in shoot number per monthly culture passage. Within a production period less than 4 months, a commercial laboratory can obtain as many as one million plantlets of boston fern per year starting from a single explant.

Shoot tips -with the proliferation of lateral shoots, single node, axillary bud, and meristem cultures are the most acceptable methods of propagation plants in vitro to maintain the genetic integrity of a clone. And it is now applied to a wide range of ornamental plants (eg, rose, gerbera, carnation, phlox, orchid and many species of woody plants ( eg, Eucalyptus, Melalua, Morus spp, etc.…..)

In this course the techniques that can be applied to ornamental plants propagated in vitro will be describe.

1. Shoot-tip culture

2. Meristem-tip culture

3. Single node culture

4. Axillary bud culture

5. Micrografting