Antibiofilm and antivirulence potential of silver nanoparticles against multidrug-resistant Acinetobacter baumannii

Faculty of Medicine

Assiut University

Antibiofilm and antivirulence potential of silver nanoparticles against multidrug-resistant Acinetobacter baumannii

Research Authors
Helal F Hetta, Israa Al-Kadmy, Saba Saadoon Khazaal, Suhad Abbas, Ahmed Suhail, Mohamed A El-Mokhtar, Noura H Abd Ellah, Esraa A Ahmed, Rasha B Abd-Ellatief, Eman A El-Masry, Gaber El-Saber Batiha, Azza A Elkady, Nahed A Mohamed, Abdelazeem M Algammal
Research Date
Research Department
Department of Microbiology and Immunology
Research Journal
Scientific reports
Research Member
Helal Fouad Hetta Hussein
Nahed Ahmed Mohamed Mohamed Abdelaal
Rasha Bakheet Abd-ellatief Mohamed
Mohamed Ahmed El-Mokhtar
Research Publisher
Nature Publishing Group
Research Year
2021
Research_Pages
1-11
Research Abstract

We aimed to isolate Acinetobacter baumannii (A. baumannii) from wound infections, determine their resistance and virulence profile, and assess the impact of Silver nanoparticles (AgNPs) on the bacterial growth, virulence and biofilm-related gene expression. AgNPs were synthesized and characterized using TEM, XRD and FTIR spectroscopy. A. baumannii (n = 200) were isolated and identified. Resistance pattern was determined and virulence genes (afa/draBC, cnf1, cnf2, csgA, cvaC, fimH, fyuA, ibeA, iutA, kpsMT II, PAI, papC, PapG II, III, sfa/focDE and traT) were screened using PCR. Biofilm formation was evaluated using Microtiter plate method. Then, the antimicrobial activity of AgNPs was evaluated by the well-diffusion method, growth kinetics and MIC determination. Inhibition of biofilm formation and the ability to disperse biofilms in exposure to AgNPs were evaluated. The effect of AgNPs on the expression …