Dr. Waleed Ahmed El Sayed Ahmed

Associate Professor, Department of Chemistry , Faculty of Science, ِِAssiut University

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Educational Qualifications:
  • Ph. D. In Integrated Biotechnology ( Nanobiotechnology ) , Sogang University, Sogang University, 2011
  • M. Sc. In Chemistry ( Organic Chemistry ) , Faculty of Science, Assiut University, 2006
  • B. Sc. In Chemistry ( Chemistry ) , Faculty of Science, Assiut University, 1998
  • Associate Professor, Department of Chemistry , Faculty of Science, ِِAssiut University, 2016
  • Lecturer, Department of Chemistry , Faculty of Science, ِِAssiut University, 2011
  • Assistant Lecturer, Department of Chemistry , Faculty of Science, Assiut University, 2006
  • Demonstrator, Department of Chemistry , Faculty of Science, Assiut University, 1999

Research fields:
Bacteriology , Nanobiotechnology


1 - Waleed A El-Said, Jeong-woo Choi, High selective spectroelectrochemical biosensor for HCV-RNA detection based on a specific peptide nucleic acid, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, Vol. 217, pp. 288-293, 2019
2 - Ismail I Althagafi, Saleh A Ahmed, Waleed A El-Said, Fabrication of gold/graphene nanostructures modified ITO electrode as highly sensitive electrochemical detection of Aflatoxin B1, PloS one, January, 2019
3 - Amal A. Elkhawaga,Marwa M. Khalifa,Omnia El-badawy,Mona A. Hassan,Waleed A. El-Said, Rapid and Highly Sensitive Detection of Pyocyanin Biomarker in Different Pseudomonas aeruginosa Infections using Gold Nanoparticles Modified Sensor, PLOS ONE , pp. 1-16,, 2019
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4 - Marwa M. Khalifa, Amal A. Elkhawaga, Mona A. Hassan, Asmaa M. Zahran, Ahmed M. Fathalla, Waleed A. El-Said & Omnia El-Badawy , Highly Specific Electrochemical Sensing of Pseudomonas aeruginosa in Patients Suffering from Corneal Ulcers: A Comparative Study, Scientific Reports , Vol. 9, 18320, pp. 1-12,, 2019
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5 - Waleed A El‐Said, Mona A Abdel‐Rahman, Eman M Sayed, Aboel‐Magd A Abdel‐Wahab, Electrochemical Monitoring of Methotrexate Anticancer Drug in Human Blood Serum by Using in situ Solvothermal Synthesized Fe3O4/ITO Electrode, Electroanalysis, Vol. 31 - No. 5, pp. 829-837, 2019
6 - Waleed A. El-Said, M. Abdel-Shakour, Alaa M. Abd-Elnaiem, An efficient and low-cost photoanode for backside illuminated dye-sensitized solar cell using 3D porous alumina , Materials Letters , Elsevier, Vol. 222, pp. 126–130,, March, 2018
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7 - Ramadan A. Mohamed1 Waleed A. El-Said2 Ahmed K. Ibrahim, Fungal biodiversity in sewage water under the effect of calcium hydroxide and hydrogen peroxide into two-steps treatment, Int. J. Environ. Sci. Technol , Springer, 15, 5, 957-967,, May, 2018
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8 - El-Din A.F.T., El-Khouly M.E., Elshehy E.A., Atia A.A., El-Said W.A., Cellulose acetate assisted synthesis of worm-shaped mesopores of MgP ion-exchanger for cesium ions removal from sea water, Microporous and Mesoporous Materials, 265, pp. 211-218, 2018
9 - El-Said W.A., Moharram A.S., Hussein E.M., El-Khawaga A.M., Design, synthesis, anticorrosion efficiency, and applications of novel Gemini surfactants for preparation of small-sized hollow spheres mesoporous silica Nanoparticles, Materials Chemistry and Physics , Vol. 211, pp. 123-136, 2018
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10 - El-Said W.A., El-Khouly M.E., Ali M.H., Rashad R.T., Elshehy E.A., Al-Bogami A.S., Synthesis of Mesoporous Silica-Polymer Composite for the Chloridazon Pesticide Removal from Aqueous Media, Journal of Environmental Chemical Engineering, 6, pp. 2214–2221, 2018
11 - El-Said W. A., Moharram A. S., Hussein E. M., El-Khawaga A. M., Synthesis, Characterizations and Applications of Some New Trimeric-Type Cationic Surfactants, Journal of Surfactants and Detergents, DOI: 10.1002/jsde.12041, 2018
12 - Abdelreheem Abdelfatah Saddik Adel Mohamed Kamal El‐Dean Waleed Ahmed El‐Said Khairy Mohamed Hassan Mohamed Saad Abbady, Synthesis, Antimicrobial, and Anticancer Activities of a New Series of Thieno[2,3‐d] Pyrimidine Derivatives , Journal of Heterocyclic Chemistry , Wiley, 55, 2111-2122,, July, 2018
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13 - AbdElazim M. Mebed, Alaa M. Abd-Elnaiem, Waleed El-Said, Tesleem Asafa, Review on Formation of Anodic Metal Oxides and their Sensing Applications , Current Nanoscience , Bentham Science, 14, 10.2174/1573413714666180817130835, 8, 2018
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14 - Waleed A. El‑Said1, Jinho Yoon and Jeong‑Woo Choi , Nanostructured surfaces for analysis of anticancer drug and cell diagnosis based on electrochemical and SERS tools, Nano convergence, December, 2018
15 - Waleed A. El-Said, Kawthar Abd El-Hameed, Nagwa Abo El-Maali, Hayam G. Sayyed, Label‐free Electrochemical Sensor for Ex‐vivo Monitoring of Alzheimer's Disease Biomarker, Electroanalysis, Vol.29, PP.748-755, March, 2017
16 - Waleed Ahmed El-Said, Hyeon-Yeol Cho, Jeong-Woo Choi , SERS Application for Analysis of Live Single Cell, Nanoplasmonics-Fundamentals and Applications, Gregory Barbillon (Ed), ISBN 978-953-51-3278-3, Print ISBN 978-953-51-3277-6, DOI: 10.5772/67593, Chapter 16, pp. 361-398, 2017
17 - Dina Fouad, Waleed El-Said, Mohamed Hussin, Mohamed Elgahami, Silica-gold nanocomposite for removal of organophosphorous pesticides , Plasmonics, 12(3), pp. 869–875, 2017
18 - Waleed A. El-Said, Dina Fouad, Mohamed Hussin, Mohamed Elgahami, Green synthesis of magnetic mesoporous silica nanocomposite and its adsorptive performance against organochlorine pesticides , International Journal of Environmental Science and Technology, DOI: 10.1007/s13762-017-1530-9, 2017
19 - Moemen A. Mohamed, Fatma M. Mohamed and Waleed A. El-Said, Enhancement of Antimicrobial Sensitivity of Salmonella and Escherichia coli Strains Isolated from Chickens Using Silver Nanoparticles in Assiut Governorate, Zagazig Veterinary Journal, 45(3), pp. 273-282, 2017
20 - Jae-Wook Shin, Kyeong-Jun Kim, Jinho Yoon, Jinhee Jo, Waleed Ahmed El-Said and Jeong-Woo Choi, Silver Nanoparticle Modified Electrode Covered by Graphene Oxide for the Enhanced Electrochemical Detection of Dopamine, Sensors, 17, pp. 2771, 2017
21 - M A Osman, Waleed A El-Said, A A Othman and A G Abd-Elrahim, Influence of thermally induced structural and morphological changes, and UV irradiation on photoluminescence and optical absorption behavior of CdS nanoparticles, Journal of Physics D: Applied Physics , IOP, 49, 165302,, March, 2016
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22 - Waleed A. El-Saida, Dina M. Fouada, Sherif A. El-Safty, Ultrasensitive label-free detection of cardiac biomarker myoglobinbased on surface-enhanced Raman spectroscopy, Sensors and Actuators B: Chemical, 228, 401-409, January, 2016
23 - Ahmed H. Osman, Waleed A. El-Said and M. Abd El-Shakour, Synthesis and characterization of some new ruthenium (II) complexes as photosensitizers in dye-sensitized solar cells, Journal of Advances in Chemistry, Volume 12 Number 5, 4413-4426,, April, 2016
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24 - Dina M. Fouad and Waleed A. El-Said, Selective Electrochemical Detection of Epinephrine Using Gold Nanoporous Film, Journal of Nanomaterials , pp. 1 - 8, 2016
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25 - Waleed A. El-Said and Dina M. Fouad, Size and Morphological Controlled of Gold Nanoparticles based on Deposition Time, Trends in Nanotechnology & Material Science, 1, pp. 1-5, 2016
26 - Waleed Ahmed El-Said, Tae-Hyung Kim, Yong-Ho Chung, Jeong-Woo Choi, Fabrication of new single cell chip to monitor intracellular and extracellular redox state based on spectroelectrochemical method, Biomaterials, Elsevier, 40, 80–87,, February, 2015
27 - D. M. Fouad, W.A.Said, M.B.Mohamed, Spectroscopic characterization of magnetic Fe3O4@Au core shell nanoparticles , Spectrochimica Acta Part A, 140, 5, 2015
28 - M A Osman A A Othman Waleed A. El-Said A G Abd-Elrahim A A Abu-sehly, Thermal annealing and UV irradiation effects on structure, morphology, photoluminescence and optical absorption spectra of EDTA-capped ZnS nanoparticles, Journal of Physics D: Applied Physics , IOP, 49, 055304,, December, 2015
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29 - Waleed Ahmed El-Said, Tae-Hyung Kim, Ki-Bum Lee, and Jeong-Woo Choi, Nanopatterned Surfaces for Stem-Cell Engineering , Stem Cells NanoEngineering, Wiley-Blackwell , ISBN: 978-1-118-54061-9, pp. 99-122, 2015
30 - Maii I. Sholqamy, Ehab S. Abd-ElHamid, Amr H. Mostafa El-Bolok, Ali F. Mohamed and Waleed A. El-Said, Oxidative stress as a cytotoxic effect that introduced by gold nanorods on hep-2 cell line, International Journal of Academic Research, 7(5), DOI:, 2015
31 - Naeem Akhtar, Sherif A. El-Safty, Md. Khairy, Waleed A. El-Said, Fabrication of a highly selective nonenzymatic amperometric sensor for hydrogen peroxide based on nickel foam/cytochrome c modified electrode , Sensors and Actuators B: Chemical, 207, pp. 158-166, 2015
32 - Waleed A. El-Said, Seung U. Kim, Jeong-Woo Choi, Monitoring in vitro neural stem cell differentiation based on surface-enhanced Raman spectroscopy using a gold nanostar array, J. Mater. Chem. C, 3, pp. 3848-3859, 2015
33 - Tae-Hyung Kim, Taek Lee, Waleed A. El-Said and Jeong-Woo Choi, Review Graphene-Based Materials for Stem Cell Applications, Materials, 8, pp. 8674–8690, 2015
34 - Waleed Ahmed El-Said, Jeong-Woo Choi, Electrochemical Biosensor consisted of conducting polymer layer on gold nanodots patterned Indium Tin Oxide electrode for rapid and simultaneous determination of purine bases, Electrochimica Acta, January, 2014
35 - Alaa M. Abd-Elnaiem, A.M. Mebed, Waleed Ahmed El-Saidd, M.A. Abdel-Rahim, Porous and mesh alumina formed by anodization of high purity aluminum films at low anodizing voltage, Thin Solid Films , Elsevier, 570, 49–56,, November, 2014
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37 - Jin-Ho Lee, Waleed A. El-Said, Byung-Keun Oh and Jeong-Woo Choi, Enzyme-free glucose sensor based on Au nanobouquet fabricated indium tin oxide electrode, J. Nanosci. Nanotechnol., 14, pp. 8432-8438, 2014
38 - Waleed A. El-Said and Jeong-Woo Choi, In situ Detection of Neurotransmitter Release from PC12 cells using Surface Enhanced Raman Spectroscopy, Biotechnol. Bioprocess Eng., 19, pp. 1069-1076, 2014
39 - Elham S. Aazam, Waleed A. El-Said, Synthesis of New Schiff-base Metals Complexes, Copper/Nickel Nanoparticles and their Cytotoxicity/Catalytic Activities, Bioorganic Chemistry Journal, 57, pp. 5–12, 2014
40 - Waleed A. El-Said, Hyeon-Yeol Cho, Cheol-Heon Yea, Jeong-Woo Choi, Synthesis of Metal Nanoparticles Inside Living Human Cells Based on the Intracellular Formation Process, Advanced Materials, DOI: 10.1002/adma.201303699, PP. 1-9, December, 2013
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41 - Tae-Hyung Kim, Waleed A. El-Said, Jeong-Woo Choi, ITO/Gold nanoparticle/RGD peptide Composites to Enhance Electrochemical Signals and Proliferation of Human Neural Stem Cells, Nanomedicine: Nanotechnology, Biology and Medicine, 9, pp. 336-344, 2013
42 - Taek Lee, Yong-Ho Chung, Qi Chen, Waleed A. El-Said, Junhong Min, Jeong-Woo Choi, Nanoscale Biofilm Modification-Method Concerning a Myoglobin/11-MUA Bilayers for Bioelectronic Device, Nanosci. Nanotechnol. , PP. 4119-4126, 2012
43 - Jeung Hee An, Waleed A. El-Said and Jeong-Woo Choi, Cell chip based monitoring of toxic effects on dopamingergic cell, J. Nanosci. Nanotechnolo., 12, pp. 4115-4118, 2012
44 - Tae-Hyung Kim, Waleed A. El-Said, Jeong-Woo Choi, Highly Sensitive Electrochemical Detection of Potential Cytotoxicity of CdSe/ZnS Quantum Dots Using Neural Cell Chip , Biosensors and Bioelectronics , 32, pp. 266–272, 2012
45 - Md. Abdul Kafi, Waleed A. El-Said, Tae-Hyung Kim, and Jeong-Woo Choi, Cell adhesion, spreading, and proliferation on surface functionalized with RGD nanopillar arrays , Biomaterials, 33, pp. 731-739, 2012
46 - Waleed Ahmed El-Said, Tae-Hyung Kim, Cheol-Heon Yea, Hyuncheol Kim and Jeong-Woo Choi, Fabrication of Gold Nanoparticle Modified ITO Substrate to Detect β-Amyloid Using Surface-Enhanced Raman Scattering, Nanosci. Nanotechnol , PP. 768-772, 2011
47 - Jeung-Hee An, Waleed Ahmed El-Said and Jeong-Woo Choi , Surface Enhanced Raman Scattering of Neurotransmitter Release in Neuronal Cells Using Antibody Conjugated Gold Nanoparticles, Nanosci. Nanotechnol, Vol. 11, PP. 1585-1588, 2011
48 - Jeung-Hee An, Waleed Ahmed El-Said, Cheol-Heon Yea, Tae-Hyung Kim and Jeong-Woo Choi , Surface-Enhanced Raman Scattering of Dopamine on Self-assembled Gold Nanoparticles, Nanosci. Nanotechnol, Vol. 11, PP. 4424-4429, 2011
49 - Rama Bhusal, Cheol-Heon Yea, Waleed Ahmed El-Said, Hyuncheol Kim and Jeong-Woo Choi , Detection of the Effect of Environmental Toxicants on the Synthetic Peptide Modified Neural Cell Chip, Nanosci. Nanotechnol, Vol .11, PP. 4605-4610, 2011
50 - Mi Jung, Waleed Ahmed El-Said and Jeong-Woo Choi, Fabrication of Nanoconcave Surface for Cell immobilization in Cell–based Chip, Nanosci. Nanotechnol, Vol.11, PP.4205-4209, 2011
51 - Mi Jung, Waleed Ahmed El-Said and Jeong-Woo Choi , Fabrication of gold nanodot arrays on a transparent substrate as a nanobioplatform for label-free visualization of living cells, Nanotechnology, 2011
52 - Junhong Min, Cheol-Heon Yea, Waleed Ahmed El-Said, Jeong-Woo Choi, The Application of Cell Based Biosensor and Biochip for Environmental Monitoring. Atmospheric and Biological Environmental Monitoring. Young J. Kim. Ulrich Platt. Man Bock Gu, Hitoshi lwahashi (Eds), PP.261-274, 2011
53 - Waleed A. El-Said, Jin-Ho Lee, Byung-Keun Oh, Jeong-Woo Choi, Electrochemical Sensor to Detect Neurotransmitter Using Gold Nano-Island Coated ITO Electrode, J. Nanosci. Nanotechnol., 11, pp. 6539-6543, 2011
54 - Waleed Ahmed El-Said, Tae-Hyung Kim, Hyuncheol Kim and Jeong-Woo Choi , Three-dimensional mesoporous gold film to enhance the sensitivity of electrochemical detection, Nanotechnology , Vol. 21, No. 45, 2010
55 - Waleed Ahmed El-Said, Tae-Hyung Kim, Hyuncheol Kim, b and Jeong-Woo Choia, Detection of effect of chemotherapeutic agents to cancer cells on gold nanoflower patterned substrate using surface-enhanced Raman scattering and cyclic voltammetry, Biosensors and Bioelectronics, Vol. 26, Issue. 4,, PP.1486-1492, December, 2010
56 - Waleed Ahmed El-Said, Jin-Ho Lee, Byung-Keun Oh and Jeong-Woo Choi, 3-D nanoporous gold thin film for the simultaneous electrochemical determination of dopamine and ascorbic acid , Electrochemistry Communications , Vol. 12, Issue. 12,, PP.1756-1759 , December, 2010
57 - Waleed Ahmed El-Said, Cheol-Heon Yea, Mi Jung, Hyuncheol Kim and Jeong-Woo Choi, Analysis of effect of nanoporous alumina substrate coated with polypyrrole nanowire on cell morphology based on AFM topography , Ultramicroscopy , Vol. 110, Issue. 6,, PP.676-681, May, 2010
58 - Waleed Ahmed El-Said, Tae-Hyung Kim, Hyuncheol Kim, Jeong-Woo Choi, Analysis of Intracellular State Based on Controlled 3D Nanostructures Mediated Surface Enhanced Raman Scattering , plosone Open Access Freely available online, 2010
59 - Taek Lee, Waleed Ahmed El-Said, Junhong Min, Byung-Keun Oh, Jeong-Woo Choi,, Verification of surfactant CHAPS effect using AFM for making biomemory device consisting of recombinant azurin monolayer, Ultramicroscopy, Vol. 110, No.6, PP. 712-717, 2010
60 - Waleed Ahmed El-Said, Cheol-Heon Yea, Hyunhee Kim, Byung-Keun Oh and Jeong-Woo Choi, Cell-based chip for the detection of anticancer effect on HeLa cells using cyclic voltammetry, Biosensors and Bioelectronics, Vol. 24, Issue. 5, , PP.1259-1265, January, 2009
61 - Waleed Ahmed El-Said, Cheol-Heon Yea, Hyunhee Kim, Jeong-Woo Choi., Fabrication of self-assembled RGD layer for cell chip to detect anticancer drug effect on HepG2 cells., Current Applied Physics, 2009
62 - Waleed Ahmed El-Said, Cheol-Heon Yea, Il-Keun Kwon & Jeong-Woo Choi., Fabrication of Electrical Cell Chip for the Detection of Anticancer Drugs and Environmental Toxicants Effect., Biochip Journal, Vol.3, No.2, PP.105-112, 2009
63 - Waleed Ahmed El-Said, Cheol-Heon Yea, Jeong-Woo Choi, Il-Keun Kwon., Ultrathin polyaniline film coated on an indium–tin oxide cell-based chip for study of anticancer effect., Thin Solid Films, Vol.518, No.2, PP.661-667, 2009
64 - Cheol-Heon Yea, Bumhwan Lee, Hyunhee Kim, Sang-Uk Kim, Waleed Ahmed El-Said, Junhong Minc, Byung-Keun Oh and Jeong-Woo Choi., The immobilization of animal cells using the cysteine-modified RGD oligopeptide, Ultramicroscopy, Vol.108, PP.1144– 1147, 2008

Supervisions :

1- ِAhmed Galal Abd-Elrahim Rashwan, Influence of Thermally induced Structural and Morphological Changes, and UV Irradiation on Photoluminescence and Optical Absorption Behavior of ZnxCd1-xS Nanocompounds, M. Sc, 28 February 2016
2- Mohamed Hussien, Synthesis, Modified of some new Mesoporous silica nanostructure and their applications in environmental impact, M. Sc, 17 February 2018

Awards and Grants :

1 - " The State Encouragement Prize " , 2016.
Number of courses0 course