In Egypt, grapes, Vitis vinifera are an important agricultural export crop. The use of various synthetic pesticides is essential to control diseases and pests for export purposes. Pesticide residues are a significant concern for food safety and quality in the Egyptian grape industry. This study evaluated the concentrations of pesticide residues in grape samples taken from five local markets in Assiut Governorate using LC-MS/MS. Fourteen types of pesticide residues were identified. The data from this study indicated that some pesticide residues exceeded the maximum residue limit set by regulatory authorities. These pesticide residues included carbendazim, buprofezin, pendimethalin, metolachlor, thiamethoxam, clothianidin, dimethoate, and omethoate. Continuous research and policy actions are necessary to ensure the safety of the food supply. The study emphasized the need for improved pesticide management, oversight, and farmer education to ensure food safety and reduce the risk of harmful pesticide residues being consumed through grape consumption. Suggestions were made to strengthen legal frameworks and promote integrated pest management strategies in the grape industry.