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Characterization of thermo/halo stable cellulase produced from halophilic Virgibacillus salarius BM-02 using non-pretreated biomass

Research Authors
Naeima MH Yousef, Asmaa MM Mawad
Research Abstract

The production of extremozymes from halophilic bacteria has increased significantly due to their stability and efficiency in catalyzing a reaction, as well as their capacity to display optimum activity at various salt concentrations. In the current study, the halophilic bacterium Virgibacillus salarius strain BM-02 could utilize many non-pretreated substrates including cellulose, corn stover, sugarcane bagasse and wheat bran as a sole carbon source. However, wheat bran was the best substrate for achieving optimum saccharification yield (90.1%). The partially purified cellulase was active and stable at a wide range of pH (5–8) with residual activities> 58%. Moreover, it was stable at 5–12% of NaCl. Metal ions have a variable impact on the activity of partially purified cellulase however, Fe+ 3 exhibited the highest increase in the cellulase activity. The enzyme exhibited a thermal stability at 40, 50 and 60 C with half-lives of

Research Date
Research Journal
World Journal of Microbiology and Biotechnology
Research Publisher
Springer Netherlands
Research Year
2023
Research Pages
1-13