Root-knot nematodes pose a severe threat to worldwide agricultural development. Due to the high
toxicity of chemical nematicides, eco-friendly control strategies against root-knot nematodes need
to be established. A pot and in vitro experiment were performed to estimate nematicidal potential
of Pochonia chlamydosporia. P. chlamydosporia was used alone or in combination with two
botanicals for controlling Meloidogyne incognita in chickpea. The laboratory assessment was
performed with four prepared concentrations (S, S/2, S/10, S/25) of fungal inoculum of
P. chlamydosporia against egg hatching and second-stage juvenile’s mortality of M. incognita. All
four concentrations reduced egg hatching and increased mortality of J2s. In pot experiment, P.
chlamydosporia was used with chopped leaves of two botanicals viz., Ageratum conyzoides and
Eichhornia crassipes against M. incognita in chickpea. All the treatments found significantly
suppressed root infestation caused by M. incognita and improved growth and physiological
attributes of chickpea. The combined application of P. chlamydosporia + A. conyzoides was found
highly effective, and E. crassipes alone was least. Therefore, using P. chlamydosporia with
botanicals is a promising sustainable strategy in agriculture against M. incognita infected chickpea