Skip to main content

New burn model for developing consistent second- and third-degree burn injuries in rats

Research Authors
Ahmed Ibrahim, Khaled M. A. Hassanein, Mahmoud Soliman, Abdelnaby M. Elshahawy
Research Member
Research Date
Research Year
2025
Research Journal
BMC Research Notes
Research Publisher
Springer nature
Research Vol
1
Research Rank
Q1
Research_Pages
1-6
Research Website
https://doi.org/10.1186/s13104-025-07200-8
Research Abstract

Abstract
Objective This study’s aim was: (1) introduce the digital drying oven as a reproducible, controllable, and accurate
heating device for burn model creation. (2) Define the heating temperature appropriate for developing consistent
second and third-degree burn injuries in rats.
Results Burns appeared deeper with more distinct borders in groups (B) and (C) than in group (A). The stainless-steel
rod at 100 ºC created burn injuries of the second degree, evidenced by the sloughing of the epidermis and necrosis
in the epithelium and upper part of the dermis. Heating at 150 and 200 ºC created third-degree burn injuries, where
necrosis involved the epidermis and dermis and extended to the subcutaneous fat and muscles. The depth of the
burn wound in the group (B) (371.2 ± 41.3 μm) and (C) (385.2 ± 38.0 μm) was significantly deeper compared with the
group (A) (178 ± 46.6 μm) (P < 0.001). The digital drying oven is a reliable, reproducible, and controllable heating device
for creating burn models. The stainless-steel rod (63 g and 8 mm) heated at 100 and 150 ºC with a contact time of 30 s
is adequate for creating consistent second and third-degree burn injuries in rats, respectively.