هل لديك سؤال ؟
تاريخ البحث
قسم البحث
مستند البحث
مجلة البحث
Egyptian Journal of Medical Microbiology
المشارك في البحث
الناشر
the Egyptian society of medical microbiology
عدد البحث
34
موقع البحث
https://ejmm.journals.ekb.eg/article_411657.html
سنة البحث
2025
ملخص البحث
Background: Double-positive (DP) and double-negative (DN) thymocytes are well-characterized stages of T cell development in the thymus. Disturbances in their levels have been previously observed in chronic inflammatory diseases and autoimmune disorders. Objective: This study aimed to analyze follicular DP and DN T cell levels in ulcerative colitis patients' peripheral blood and colon tissue biopsy and determine whether their levels correlate with disease severity. Methodology: This study involved 22 patients with ulcerative colitis (UC) and 18 healthy controls. Flow cytometry was used to measure the total levels of DP and DN T cells and their subsets in each participant's peripheral blood and colon tissue biopsy. Results: DP T cells were higher in tissue than in the blood samples. DN T cells were lower in tissue than the blood but higher in patients than control tissues. Differences were observed in the tissue levels of follicular DP and DN cells and their expression levels of CD154 compared with blood and in severe compared with non-severe cases. Conclusion: Levels of DP and DN follicular T cells are higher in the tissue of UC patients than in blood, with higher expression of CD154 in tissues. Levels of these cells are higher in more severe cases and show good efficiencies in predicting disease severity. Further research is needed to assess whether these cells have a role in the inflammatory process in UC.
