The effect of in vivo exposure to roridin E alone and in combination with linoleic acid on rats was studied. Treatment of rats with a sublethal dose of roridin (2 mg/kg body weight) alone did not caused an significant effect on most studied biochemical parameters in liver and kidney tissues and plasma. However, co-administration of linoleic acid with roridin E resulted in increasing its toxicity as evidenced by the the biochemical changes.