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First light microscopy and ultrastructural description of Mesocoelium sociale (Luhe, 1901) Odhner, 1910 (Trematoda: Mesocoeliidae) in Bufo regularis from Egypt

Research Abstract

Background

Mesocoeliidae is a common parasite of the small intestine of amphibians and reptiles. From Egypt, only Mesocoelium monas (Rudolphi C., Berolini 811 (1819)) (Freitas JF, Revista Brasiliera de Biologia 18:171-174, (1958)) was reported and described by (Saad AI et. al., J Egypt Ger Soc Zool 33: 219-234, (2000)) from the small intestine of the Egyptian toads Bufo regularis from Aswan Governorate, Upper Egypt, and the molecular characterization of the same parasite was reported by (Mansour MFA et. al., Egypt. J. Biol. (Zool.),10:(1)1-8, (2014)) without any morphological description from the same final host. The present study aimed to detect the prevalence and the characteristic morphological features of mesocoeliid parasites of the Egyptian toads Bufo regularis.

Methods

Toads were collected from Assiut and Giza Governorates during the autumn of 2018. They were dissected and examined by a dissecting microscope for intestinal parasites. The collected trematodes were fixed, stained, dehydrated, cleared, and mounted. Photomicrographs were taken and parasite measurements were determined. Some of the identified worms were prepared for scanning electron microscope for studying their ultrastructure.

Results

Mesocoelium sociale was detected from the small intestine of 14 out of 51 toads Bufo regularis (27.5%) with worm burden (10–30 worms per host). Detailed morphometric characteristic features of the parasite were described by a light microscope. Scanning electron microscopy showed many fine ultrastructure details in the present study and confirmed the light microscopic description.

Conclusion

Mesocoelium sociale (Luhe M, Centralblatt für Bakteriologie Parasitenkunde und Infektionsk-rankheiten, 30, 166-177, (1901)) (Odhner T., Res. Swedish Zool Exped Egypt iv, (23a), 1–166, (1910)) is recorded for the first time from Egypt as a trematode parasite of the Egyptian toads’ Bufo regularis and described by a light microscope. In addition, it is described for the first time worldwide by scanning electron microscopy.

Research Authors
Hasnaa Thabit, Refaat Khalifa
Research Date
Research Department
Research Journal
The Journal of Basic and Applied Zoology
Research Pages
1-10
Research Publisher
Springer Berlin Heidelberg
Research Vol
81
Research Year
2020

Morphological and molecular characterization of Linguatula serrata and evaluation of the health status of the infested dogs

Research Abstract

Linguatula serrata is a neglected parasitic zoonosis with less research recorded throughout the globe on the physiological and immunological alteration of dogs infested by these zoonotic parasites. This study aimed to evaluate the effects of L. serrata infestation on the dogs after experimental infestation with the nymphs by determining oxidative stress levels and immunological genes of cellular immunity. Larvae of L. serrata were collected from the infested goat mesenteric lymph nodes by cutting it, and the nymphs were liberated; these nymphs were mixed with saline at 37–40 °C for experimental infestation of dogs and observed infestation to puppies using viable nymphal stages (movable, not traumatized or dead). Blood and sera samples were sampled from the dog after 6 months of infestation. The nasal mucosa and its mucus were collected from the infested dogs as well as negative controls for evaluation of …

Research Authors
Marwa M Attia, Olfat A Mahdy, Soliman M Soliman, Salma I El-Samannoudy, Hasnaa Thabit
Research Date
Research Department
Research Journal
Comparative Clinical Pathology
Research Pages
1-10
Research Publisher
Springer London
Research Year
2023

Coinfection of the gut with protozoal and metazoal parasites in broiler and laying chickens

Research Abstract

The chicken business faces substantial economic losses due to the risk of parasitic coinfection. Because the current study aimed to investigate enteric parasitic coinfections problems among the suspected examined chicken farms, samples were collected during the field investigation from suspected freshly dead birds, clinically diseased, apparently healthy, and litter samples for further laboratory parasitological, histopathological, and immunological examinations. Variable mortalities with various clinical indicators, such as ruffled feathers, weight loss, diarrhea of various colors, and a decline in egg production, occurred on the farms under investigation. In addition, the treatment protocols of each of the farms that were evaluated were documented and the m-RNA levels of some cytokines and apoptotic genes among the infected poultry have been assessed. The prevalence rate of parasitic coinfection in the current …

Research Authors
Mohamed R Mousa, Marwa M Attia, Heba M Salem, Nawal Al-Hoshani, Hasnaa Thabit, Marwa A Ibrahim, Haleema H Albohiri, Samar Ahmad Khan, Mohamed T El-Saadony, Khaled A El-Tarabily, Mohamed A El-Saied
Research Date
Research Department
Research Journal
Poultry Science
Research Pages
103227
Research Publisher
Elsevier
Research Vol
103
Research Year
2024

The Biological Activity of Illicium verum (Star Anise) on Lernaea cyprinacea-Infested Carassius auratus (Goldfish): In Vivo Study

Research Abstract

Lernaea cyprinacea infestation is considered a serious economic problem in the fish market. An assessment to control this parasite is needed to manage this problem. The Illicium verum oil extract has considerable antioxidant activity and scavenges 96.22% of free radicals; the high antioxidant activity refers to the phenolic content presence. The extract contains minerals, especially K, fibers, and dry matter. So, the Illicium verum ingredients were tested against this copepod for in vitro and in vivo investigation with the assessment of the treatment trial using a scanning electron microscope and evaluating the change in different immunological genes in goldfish. Female parasitic L. cyprinacea worms were blackish and hairy. The in vitro study on L. cyprinacea adults using star anise revealed that the LC50 was 12.5 and 25 μg/mL for 2 and 1 h exposure periods, respectively. Interleukin (IL-1β) and IL-6 were grossly upregulated in C. auratus-infested skin by L. cyprinacea after treatment by 1 week, then declined after 3 weeks. In contrast, TNF-α was 18 folds upregulated in the first week after treatment, with a decline after 3 weeks. In conclusion, star anise is recommended as a safe and economical agent for controlling L. cyprinacea infestation in fish.

Research Authors
Marwa M. Attia, Amal M. Alzahrani, Magdy I. Hanna, Heba M. Salem, Mohammed A. S. Abourehab, Mohamed T. El-Saadony and Hasnaa Thabit
Research Date
Research Department
Research Journal
Life
Research Pages
2054
Research Publisher
MDPI
Research Vol
12
Research Year
2022

The immune responses of Oreochromis niloticus against Prohemistomum vivax encysted metacercariae infection with the evaluation of different biomarkers stressors

Research Abstract

This study aimed at evaluating the immunological status of Oreochromis niloticus (O. niloticus); so, a total of 120 O. niloticus were collected from different farms located in Kafr El-Sheikh Governorate in Egypt during the period from January 2021 to January 2022. The fish were surveyed for commonly encysted metacercariae present in different organs such as gills, spleen, liver, kidney, and muscles. The collected encysted metacercariae were of the family Cyathocotylidae (Prohemistomum vivax) with a prevalence of 25%. Different cell-mediated immune responses such as Major histocompatibility class II alpha (MHC-IIα), Toll-like receptor 7 (TLR-7), Interleukin (IL-8), and Clusters of differentiation 4 (CD4) were assessed in different organs such as gills, spleen, liver, kidney, and muscles which revealed an elevation in different genes in infected organs as a reaction from the body against parasitic infection. In addition …

Research Authors
Nehal A Younis, Hasnaa Thabit, Salma I El-Samannoudy, Marwa M Attia
Research Date
Research Department
Research Journal
Scientific Reports
Research Pages
11885
Research Publisher
Nature Publishing Group UK
Research Vol
13
Research Year
2023

A novel adaptive synchronization algorithm for a general class of fractional-order complex-valued systems with unknown ... and color image encryption

Research Abstract

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Research Authors
A. Shoreh and G.M. Mahmoud
Research Date
Research Department
Research Journal
Physica Scripta. Volume: 99. Issue: 2. 2024 (Jan. 5, 2024)
Research Member
Research Year
2024

Complex Rayleigh–van-der-Pol–Duffing Oscillators: Dynamics, Phase, Antiphase Synchronization, and Image Encryption

Research Abstract

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Research Authors
Asma Al Themairi , Gamal M. Mahmoud , Ahmed A. Farghaly and Tarek M. Abed-Elhameed
Research Date
Research Department
Research Journal
Fractal Fract. 2023, 7, 886. https://doi.org/10.3390/fractalfract7120886
Research Rank
Q1
Research Year
2023

A novel adaptive synchronization algorithm for a general class of fractional-order complex-valued systems with unknown parameters, and applications to circuit realization and color image encryption

Research Abstract

This article proposes an adaptive synchronization (AS) algorithm to synchronize a general class of fractional-order complex-valued systems with completely unknown parameters, which may appear in physical and engineering problems. The analytical and theoretical concepts of the algorithm rely on the mathematical framework of the Mittag-Leffler global stability of fractional-order systems. A specific control system is established analytically based on the fractional-order adaptive laws of parameters, and the corresponding numerical results are executed to verify the accuracy of the AS algorithm. The proposed synchronization method is evaluated using the fractional-order complex Rabinovich system as an attractive example. The electronic circuits of the new system with different fractional orders are designed. By utilizing the Multisim electronic workbench software, various chaotic/hyperchaotic behaviors have been observed, and a good agreement is found between the numerical results and experimental simulation. In addition, the approximation of the transfer function for different fractional-order are presented. And the corresponding resistor and capacitor values in the chain ship model (CSM) are estimated, which can be utilized in designing electronic circuits for other fractional-order systems. Furthermore, two strategies for encrypting color images are proposed using the AS algorithm and fractional-order adaptive laws of parameters. In the first strategy, the color image is treated as a single package and divided into two vectors. The first vector is embedded into transmitter parameters, while the second vector is injected into the transmitter state signals. In the second strategy, the primary RGB channel components of the original color image are extracted and separated into two vectors, and the same process is followed as in the first strategy. These strategies complicate the decryption task for intruders. Different scales of white Gaussian noise are added to color images to examine the robustness of the proposed color images encryption strategies

Research Authors
A. Shoreh and G.M. Mahmoud
Research Date
Research Department
Research Journal
Physica Scripta. Volume: 99. Issue: 2. 2024 (Jan. 5, 2024).
Research Member

Complex Rayleigh–van-der-Pol–Duffing Oscillators: Dynamics, Phase, Antiphase Synchronization, and Image Encryption

Research Abstract

: This paper introduces the complex Rayleigh–van-der- Pol–Duffing oscillators (RVDOs), which are hyperchaotic and can be autonomous or nonautonomous. The fundamental dynamics of the autonomous and nonautonomous complex RVDOs, including dissipation, symmetry, fixed points, and stability, are studied. These oscillators are found in various necessary fields of physics and engineering. The paper proposes a scheme to achieve phase synchronization (PS) and antiphase synchronization (APS) for different dimensional models. These kinds of synchronization are considered a generalization of several other types of synchronization. We use the active control method based on Lyapunov’s stability theory for this scheme. By analytically determining the control functions, the scheme achieved PS and APS. Our scheme is applied to study the PS of hyperchaotic behaviors for two distinct hyperchaotic nonautonomous and autonomous complex RVDOs. Additionally, the scheme is employed to achieve the APS of a chaotic real nonautonomous RVDO and a hyperchaotic complex autonomous RVDO, including those with different dimensions. Our work presents numerical results that plot the amplitudes and phases of these hyperchaotic behaviors, demonstrating the achievement of the PS and APS. The encryption and decryption of grayscale images are researched based on APS. The experimental results of image encryption and decryption are computed with information entropy, visual analysis, and histograms.

Research Authors
Asma Althemairi, G.M. Mahmoud, Ahmed A. Farghaly, Tarek M. Abed-Elhameed
Research Date
Research Department
Research Journal
Fractal Fract. 2023 (Dec.15,2023), 7, 886. https://doi.org/10.3390/fractalfract7120886.
Research Member
Research Rank
Q1

Orogenic lode-gold deposits and listvenization processes in the El-Barramiya area, Eastern Desert, Egypt

Research Abstract

Gold mineralization in the El-Barramiya region of the Eastern Desert, Egypt, is connected to the post-accretionary stage throughout the Central Eastern Desert. It is represented by quartz, quartz-carbonate veins and disseminations in listvenite rocks. The thrust contact between rock units in El-Barramiya area played an imperative part in gold mineralization where the obduction of ophiolitic rocks over the metasediments and metavolcanics caused shear zones. Mineralization in the study area formed along shear zones and the gold mineralization prefers to precipitate along the transition zone between low-grade regional metamorphic area which is represented by metasediments and high grade which is represented by actinolite schist. Gold mineralization lode of El-Barramiya gold mine area is situated in EW trending quartz and quartz-carbonate veins along a shear zone located in the intersections between faults trend in NE-SW (Najd fault), NW-SE and thrust faults trend in NEE-SWW in metavolcanic and metasedimentary host rocks. Porphyry granite in the mine area played an important role in hydrothermal alteration process where it represents the source of K, listvenite formed when fluids rich in CO 2 and bearing-K permeate and alter the previously altered ultramafic rocks, usually serpentinites of the ophiolitic mélange rocks. Listvenitization process includes silicification and carbonatization metasomatic processes, tectonized serpentinites are altered to listvenite as the carbonatization becomes more intense close to dipping transpressive faults. Geochemical studies of listvenite and mineralized veins helped to determine the ultramafic genesis of …

Research Authors
Mohamed Abdelrady, Hany Elhadek, Mohamed Abdelmoneim, Ahmed Saleh
Research Date
Research Department
Research Journal
Environmental Earth Sciences
Research Publisher
Environmental Earth Sciences
Research Year
2023
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