In the present paper we introduce and study pre-T₀-, pre-R₀-, pre-T₁-, pre-R₁-, pre-T₂ (pre-Hausdorff)-, pre-T₃ (pre-regularity)-,  pre-T₄ (pre-normality), )-, pre-strongT₃- and pre-strong T₄ -separation axioms in fuzzifying topology and
give some of their characterizations as well as the relations of these axioms and other separation axioms in fuzzifying topology introduced by Shen [7].