Relationship of transitional regulatory B and regulatory T cells and immunosuppressive drug doses in stable renal transplant recipients

South Egypt Cancer Institute

Assiut University

Relationship of transitional regulatory B and regulatory T cells and immunosuppressive drug doses in stable renal transplant recipients

Research Authors
Eman H Ibrahim, Mostafa Aly, Christian Morath, Douaa M Sayed, Naruemol Ekpoom, Gerhard Opelz, Caner Süsal, Volker Danie
Research Member
Douaa Mohamed Sayed Hussein
Eman Hosny Mohammed Ibrahim
Research Department
Department of Clinical Pathology
Research Date
Research Year
2021
Research Journal
Immunity, Inflammation and Disease
Research Abstract

Objectives

Regulatory B cells (Bregs) and T cells (Tregs) are thought to be involved in the regulation of graft acceptance in renal transplant recipients. However, mechanisms that affect Breg differentiation and interaction with Tregs are rather unclear.

Methods

Using eight‐color‐fluorescence flow cytometry, Tregs and CD19+ CD24hiCD38hi Bregs were analyzed in whole blood samples of 80 stable kidney transplant recipients, 20 end‐stage renal disease (ESRD) patients and 32 healthy controls (HC). In addition, differentiation of Bregs and Tregs was studied in different micromilieus using cocultures with strongly enriched B‐lymphocytes and autologous peripheral blood mononuclear cells stimulated with CpG and phytohemagglutinin.

Results

Bregs were higher in HC than in ESRD patients and lowest in transplant recipients. Bregs were higher early as compared to late posttransplant. Posttransplant, high Bregs