The effect of different alternative fertilizer sources in comparison with the
traditional mineral nutrition on growth characters, yield components and oil content of
Pimpinella anisum L. plant was investigated. The alternative sources used in this study
were cattle manure (CM), phosphorein, and Halex-2. Combination among these sources or
with 1/2 recommended dose of NPK chemical fertilizers were also studied. All treatments
applied significantly promoted the growth, fruit yield and volatile oil content compared to
the untreated control. The GC-MS results showed that the main components of volatile oil
were anethole and carvone. The chemical analysis of anise leaves indicate that the total
chlorophyll and carotenoids content, total carbohydrates percentage in leaves and fruits and
N, P and K percentages were significantly improves as a result of applying any fertilization
treatment compared to the control. The nitrate and nitrite of anise fruits were significantly
reduced as a result of using any alternative fertilizer treatment however; the RD treatment
recorded the highest values in this respect. The combination treatments among alternative
sources or combined with 1/2 recommended dose of NPK chemical fertilizers were more
effective than separate application. In order to obtain high quality of anise fruits and
volatile oil, decrease the production cost, protect the environment from pollution and save
the chemical fertilizers, the treatment of using Halex-2 plus phosphorein or at least adding
½ RD to Halex-2 or phosphorein bio-fertilizers was recommended. These treatments came
in the second rank after the RD of chemical fertilizer and without significant difference in
most cases.