Circulating microparticle subpopulation in metabolic syndrome: relation to oxidative stress and coagulation markers

Faculty of Medicine

Assiut University

Circulating microparticle subpopulation in metabolic syndrome: relation to oxidative stress and coagulation markers

Research Authors
Asmaa M Zahran, Sohair K Sayed, Heba A Abd El Hafeez, Walaa A Khalifa, Nahed A Mohamed, Helal F Hetta
Research Date
Research Department
Department of Clinical Pathology
Research Journal
Diabetes, metabolic syndrome and obesity: targets and therapy
Research Member
Sohair Kamel Sayed Ahmad
Research Rank
2
Research Vol
12
Research Year
2019
Research_Pages
485-493
Research Abstract

Circulating microparticles (MPs) contribute to the pathogenesis of atherothrombotic disorders and are raised in cardiovascular diseases. Herein, we aimed to investigate the effect of moderate metabolic abnormalities in an early stage of metabolic syndrome (MetS) on the level of MP subpopulations and to study relationships between MP subpopulations and both oxidative stress and coagulation markers.

Methods

Flow cytometry used to evaluate circulating MPs subpopulations in 40 patients with an early stage MetS and 30 healthy controls. ELISA was used to quantify plasminogen activator inhibitor type 1/tissue plasminogen activator (PAI-1/TPA) while plasma glutathione peroxidase (GPx) activity was measured spectrophotometrically.

Results

Total MPs were significantly elevated in MetS (P< 0.001). Glutathione peroxidase and PAI1/TPA activity was significantly increased in subjects with MetS (P< 0.001 …