ملخص البحث
Natural products play a signifcant role in providing the current demand as antiparasitic agents,
which ofer an attractive approach for the discovery of novel drugs. The present study aimed to
evaluate in vitro the potential impact of seaweed Padina pavonica (P. pavonica) extract in combating
Acanthamoeba castellanii (A. castellanii). The phytochemical constituents of the extract were
characterized by Gas chromatography–mass spectrometry. Six concentrations of the algal extract
were used to evaluate its antiprotozoal activity at various incubation periods. Our results showed that
the extract has signifcant inhibition against trophozoites and cysts viability, with complete inhibition
at the high concentrations. The IC50 of P. pavonica extract was 4.56 and 4.89 µg/mL for trophozoites
and cysts, respectively, at 24 h. Morphological alterations of A. castellanii trophozoites/cysts treated
with the extract were assessed using inverted and scanning electron microscopes and showed severe
damage features upon treatment with the extract at diferent concentrations. Molecular Docking
of extracted compounds against Acanthamoeba cytochrome P450 monooxygenase (AcCYP51) was
performed using Autodock vina1.5.6. A pharmacokinetic study using SwissADME was also conducted
to investigate the potentiality of the identifed bioactive compounds from Padina extract to be orally
active drug candidates. In conclusion, this study highlights the in vitro amoebicidal activity of P.
pavonica extract against A. castellanii adults and cysts and suggests potential AcCYP51 inhibition.
تاريخ البحث
قسم البحث
المشارك في البحث
الناشر
Scientific reports
عدد البحث
14
سنة البحث
2024
صفحات البحث
13610