Rosell ( Hibiscus sabdariffa L. is one of the most important medicinal plants. Root rot and wilt disese of roselle attack either seedling or adult plants. In this study, the isolation and identification of the pathogen associated with root rot and with symptoms of roselle in Assiut were studied during the seasons 2012-2014.The efficacy of essential oils from three plants i.e. sweet basil (Ocimum basilicum var. basilicum L.), majoram (Majoram hotinsis L.) and peppermint (Mentha piperita L.) at three concentrations (0.5, `1 and 2%) were tested against the most aggressive Fusarium isolate in vitro as well as evaluation in vtvo. Results showed that all tested isllates of Fusarium moniliforme, F. solani and three isolates of F. oxysporum (No. 1. 2 and 3) were pathogenic in roselle plants while the highest pathogenicity was caused by Fusarium oxysporum No. 3 followed by N0. 1. In vitro, the three tested oils were found to have an inhibitory effect against the growth of the pathogenic fungus, the highest inhibition was observed at 2% of majoram and peppermint (2.23 and 2.00 cm inhibition zone , respectively). Under open greenhouse conditions, results of seed coating with the three essential oils under study suppressed infection with pathogenic F. oxysporum. Majoram and peppermint were the most effective oils in reducing disease incidence, 0.5% concentration demonstrate the best protection of roselle seeds against F. oxysporum. Growth characteristics i.e. plant height, No. branches and fruits / plant, fresh and dry weighs of sepals were significantly higher at all treatments. Also, acidity and total anthocyanin increased significantly. Majoram and peppermint oils at 0.5% and basil oil at 2% were the most effective concentration in decreasing disease incidence in addition in increasing the quantity and quality of roselle yield.