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EVALUATION OF SALVIA ROSMARINUS AND CORIANDRUM SATIVUM AS CHEMOPREVENTIVE AGENTS IN INDUCED ORAL SQUAMOUS CELL CARCINOMA (AN EXPERIMENTAL STUDY)

Research Abstract
Abstract
Introduction: The most prevalent malignant tumor of the head and neck region is squamous cell carcinoma. The bad prognosis and low survival rate are mainly related to oral cancer patients. Developing chemopreventive techniques has been crucial to reduce the incidence of oral cancer. Chemoprevention with natural products has emerged as viable preventive options. Salvia rosmarinus and Coriandrum sativum have been studied and discovered to have powerful anticancer properties.
Aim of the study: The present trial was proceeded to study the effects of salvia rosmarinus and coriandrum sativum during experimental hamster buccal pouch carcinoma induction; using PCNA as cancer proliferative cell marker.
Material and Methods: A total of 60 adult male hamsters were divided into 5 equal groups. Group I, biopsies of normal pouch mucosa were obtained as a negative control. All remaining groups were painted by DMBA as a carcinogenic agent. Group II, as a positive control group, was only painted by DMBA. Group III received topically salvia rosmarinus oil. Group IV received topically coriandrum sativum oil. Group V received both oils together. After 16, 24, and 32 weeks, 2 to 4 animals from every group had been sacrificed. Tissue lesions were prepared, and paraffin sections were stained with primary antibody for PCNA immunohistochemical evaluation.
Results: The salvia rosmarinus and coriandrum sativum; either alone or in combination, significantly reduced the carcinogenic effect for DMBA-induced oral carcinogenesis.
Conclusion: Salvia rosmarinus and coriandrum sativum have a chemopreventive considerable action versus oral cancer development, and their synergetic action is considerably more effective.
Research Authors
Amr Abdelmoez email ; Hanaa Raslan; Nesrin Hassan; Asmaa Zahran; Ahmed Mohammad Hussein
Research Date
Research Department
Research Publisher
Alexandria Dental Journal
Research Website
DOI: 10.21608/ADJALEXU.2024.299564.1516
Research Year
2024

Validation of novel measurement protocols proposed for the standardized assessment of crestal bone levels: A cone-beam computed tomography study

Research Abstract

ABSTRACT

Purpose: This study was performed to introduce, evaluate, and compare various novel assessment protocols designed for straightforward, reliable, and reproducible measurement of alveolar bone levels. These protocols are intended for standardized periodontal assessment and follow-up, utilizing cone-beam computed tomography (CBCT) images and manipulation of Digital Imaging and Communications in Medicine (DICOM) viewer software.

Materials and Methods: Two experienced oral and maxillofacial radiologists developed 5 distinct radiographic measurement protocols. These techniques were established to assess the alveolar bone level of a periodontally affected upper central incisor using a method that is consistently repeatable across observers. Two additional assessors, blinded to the details of the study, independently applied the protocols to retrieved DICOM files that met the eligibility criteria. A scoring system with 3 subscores was created and used to compare the protocols.

Results: Statistically excellent inter-observer reliability was observed for all protocols, other than protocol 1, which demonstrated moderate reliability. The average discrepancy between measurements taken by the 2 observers was 1.2 mm for protocol 1, 0.81 mm for protocol 2, and less than 0.5 mm for the remaining 3 protocols. All approaches except protocol 4 were straightforward to apply.

Conclusion: This study introduces multiple reliable protocols for the evaluation of periodontal bone levels that ensure consistency across observers. Based on the findings, the double axial lines and incisocrestal distance protocols are recommended. These new assessment approaches, along with any future modifications, may be useful in periodontal assessment, dental implant follow-up, orthodontic evaluation, research, and artificial intelligence model generation. (Imaging Sci Dent 20240073)

Research Authors
Ali Fahd1,*, Aya A. Hussien2, Mohamed T. Ellabban3, Zein A. Shatat4
Research Date
Research Department
Research Journal
Imaging Science in Dentistry
Research Publisher
Imaging Science in Dentistry
Research Website
https://www.researchgate.net/publication/384429687_Validation_of_novel_measurement_protocols_proposed_for_the_standardized_assessment_of_crestal_bone_levels_A_cone-beam_computed_tomography_study
Research Year
2024
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