Flow Cytometry Analysis of DNA Ploidy and S-PhaseFraction in Salivary Gland Tumors of Egyptian Patients

Faculty of Dentistry

Assiut University

Flow Cytometry Analysis of DNA Ploidy and S-PhaseFraction in Salivary Gland Tumors of Egyptian Patients

Research Authors
Asmaa M Zahran, Hussein Fakhry, Khaled A Hussein, Mahmoud Abd El-Salam, MohamedA Mohamed, Safaa M Tohamy and Ahmed M Hussein
Research Abstract

Abstract

Aim: The aim of this study is to investigate the DNA ploidy and S-Phase Fraction (SPF) of some Salivary Gland Tumors (SGTs) in Egyptian patients and to investigate the correlation between these two biological parameters and the presumptive behavior of these neoplasms.

Methods: Flow cytometric analysis of DNA pliody and S-Phase Fraction (SPF) was done in 50 fresh tumor tissue sections of SGTs which diagnosed as 15 benign and 35 malignant tumors.

Results: 93.3% of the benign SGTs tumors were diploid, while only 34% of malignant tumors were diploid and 66% were aneuploid. The malignant SGTs had higher SPF than the benign tumors but with no significant difference. There was no significant correlation of ploidy status or SPF with the tumor grades of mucoepidermoid carcinomas.

Conclusion: DNA aneuploidy may be a key indicator for tumors activity and malignancy in SGTs, while the SPF has a minor role in the evaluation of SGTs activity.

Research Date
Research Department
Department of Oral Pathology
Research File
paper_compressed.pdf
Research Journal
Clinics in Oncology
Research Member
Safaa Marzouk Tohamy Marzouk
Research Publisher
Clinics in Oncology
Research Rank
International
Research Vol
3
Research Website
https://bit.ly/3LZ9DM8
Research Year
2018
Research Pages
1393