Gastrointestinal  parasites  have  a  direct  effect  on  the  health  of  equines.  This  study 
was  conducted  to  determine  the  prevalence  and  significance  of  gastrointestinal 
parasites  of naturally infected horses in Luxor Governorate, Egypt. A total of 100 
horse fecal samples were collected during the period from March - 2020 to February 
–  2021 from different ages, sexes and stables. All of these samples were examined 
by  different  fecal  examination  techniques  including  (direct  wet  mount, 
sedimentation and flotation techniques).  The present study investigate  that  the  total 
prevalence  of  infected  horses  was  15%.  There  is  a  relationship  between  the  GIT 
infection and the age of the horse. The highest percent of infection was recorded by 
Strongylus vulgaris  9 % followed by  Parascaris equorum  5%, while the percent of 
infection by  Balantidium coli was 1% in infected horses. It consequence that horses 
are  highly  susceptible  to  Strongylus  irrespective  of  gender  and  age  or  even 
deworming. Control measures should be put in consideration to totally overcome the 
parasitic infection.
Keywords:  Gastrointestinal  parasites,  Parascaris  equorum,  Strongylus  vulgaris, 
Balantidium coli.