Background: Gas stations have an adverse influence on the environment and workers' health due to the chemicals and physical risks they contain. So, this study aimed to assess environmental safety, stress levels and gas stations' workers' knowledge, attitude and practices regarding occupational health hazards. Subject and Method: Research Design: A descriptive cross-sectional study design. Setting: All gas stations in Assiut City (11stations) that affiliated to Ministry of Petroleum, Egypt Gas Station Directory. Subjects: 140 gas stations' workers. Tools of data collection: Three tools were utilized; Tool (1): A structure interview questionnaire that consisted of five parts; workers' personal data, sociodemographic status assessment scale, knowledge of the occupational health risks associated with gas stations, workers' reported practices and workers' attitude toward occupational health hazards. Tools (2): Assessment of the gas stations' working environment. Tools (3): Assessment of workers' stress levels. Results: It was observed that 54.3% of the workers were from urban area, their mean age ranged from 19.0 to 57.0, 64.3% of them had good knowledge with significant effect with residence, times of work and daily working hours. Also, there was effect of working hours and attainting courses on their reported practices and working times& working hours on workers' stress level. Conclusion: Less than two-thirds of gas stations' workers had high level of knowledge and had positive attitude, more than half of them had unsatisfactory reported practices and approximately two-thirds experienced moderate level of stress. Recommendations: It would be beneficial to establish ongoing training programs for gas stations' workers about occupational health hazards.