Co-administration of Glimepiride (GLIM), Empagliflozin (EMPA) and Linagliptin (LING) promote a preferred
glycemic control, besides additional benefits such as reduction of high blood pressure and weight loss. In the
present study a feasible TLC method with reflectance/ fluorescence mode for simultaneous determination of
GLM, EMPA and LING was established. Separation of the cited drugs was performed on aluminum plates precoated
with silica gel 60 F254 and developed with (toluene: methanol: ethyl acetate (4: 3: 2 v/v/v)) as a mobile
phase. The mentioned drugs were adequately detected after exposing the plate to concentrated (30–34%)
hydrochloric acid vapor for 15 min. The was scanned in the reflectance/fluorescence mode at 228 nm excitation
wavelength using 320 nm emission filter. The three drugs were well separated with Rf values of 0.73 ± 0.02,
0.59 ± 0.02 and 0.10 ± 0.02 for GLIM, EMPA and LING, respectively. The developed method was validated
according to ICH guideline with linearity (2.61–60, 5.53 –120 and 4.68–80 ng/band for GLIM, EMPA and LING,
respectively) and precision (% RSD was ± 2.98 for GLIM, 2.76 for EMPA, 2.41 for LING). The correlation coefficients
were 0.9915, 0.9940 and 0.9921 for GLIM, EMPA and LING, respectively. The developed method was
successfully applied for the analysis of the cited drugs in their tablets and real human plasma samples with good
accuracy and precision.