Abstract A field experiment was carried out at the experimental Research Station of Agric. Faculty of Assiut., Assiut Univ., Assiut Governorate. Egypt, during two successive winter seasons of 2021/2022 and 2022/2023 to study the response productivity and protein % of wheat cultivar (Giza 171) to Micobein inoculation (3 gm/L) and humic acid foliar application at a rate of (0, 2 and 3 gm./L) under nitrogen levels (25, 50 and 75 kg N/fed). The experiment was carried out in split- split plot design with four replications. The main-plots were assigned for N-fertilization, while, the sub-plots were randomly allocated for foliar application of humic acid levels. Whilst, the sub-sub plots were randomly distributed for Microbein inoculation levels (25, 50 and 75 kg/fed). The obtained results revealed that all wheat traits were significantly affected by the treatments. Inoculation have an increase percentages for heading date (HD), plant height (PL), Spike length (SL) and No. of spiks/me (SN) by (4.22 & 3.95%), (19.66 & 19.62%), (&.62 & 5.25%) and (12.82 & 13.10%) over the un –inoculated treatment respectively. Moreover, the results recorded that the application of humic acid at 3 gm /L increased markedly seed index, grain yield per fed, biological yield per fed, and % protein. The results recommended that sowing wheat Giza 171 cultivar on 15th November with inoculation with 3 gm/L of microbein and foliar application with humic acid (3gm/L) twice had a progressive effect especially with applying 50 kg N/fed., under the Assiut Governorate, Egypt.