Abstract

Aim: The current study was designed to explore the potential effect of ginger extract on the osteosarcoma cell line (MG-
63).
 

Material and Methods: Human osteosarcoma cell line (MG-63) was acquired from Nawah-Scientific. The tested drug was
ginger extract prepared by maceration in semi-liquid form. The potential impact of ginger extract upon MG-63 cells was
evaluated using cytotoxicity assay, microscopic evaluation, Enzyme-Linked Immunosorbent Assay for evaluation of Matrix
Metalloproteinase-9 (MMP-9), and Real-Time Polymerase Chain Reaction for detection of the expression levels of the proapoptotic
gene (P53) and antiapoptotic gene B-cell lymphoma 2 (B-cl2) in ginger extract-treated cells. Then the data
collected was statistically assessed.
 

Results: The outcomes demonstrated that ginger extract's cytotoxic impact upon MG-63 cells was dose-dependent, as
viability increased as long as the concentration decreased. Under the microscope, MG-63 cells treated with ginger extract
displayed apoptotic signs such as chromatin condensation, cell shrinkage, and plasma membrane blebbing. Data about
MMP-9 showed that, ginger extract dramatically and dose-dependently reduced MMP-9 levels. Regarding P53 and B-cl2,
data revealed that ginger extract significantly increased P53 levels and decreased B-cl2 in a dose-dependent manner.
 

Conclusion: Ginger extract substantially diminishes the viability of osteosarcoma cells because of its strong cytotoxic
activity against MG-63 cells.