Aim: Ulcers can weaken the oral mucosa's innate defenses. The purpose of the study was to examine the therapeutic advantages of using secretome derived from bone marrow stem cells to heal traumatic ulcers in albino rats. Materials and Methods: Thirty male albino rats with extensively damaged ulcers were randomly assigned to three groups: control group, group that received oracure gel treatment, and group that received secretome treatment. Tissues from the buccal mucosa were taken on days three, seven, and twelfth. Assessment was done via clinical evaluation, histological examination, Masson's trichrome stain, and vascular endothelial growth factor (VEGF)-specific immunohistochemistry assays. Statistical analysis was then performed on the outcomes. Results: The group treated with secretome had the greatest percentage of wound contraction and the fastest rate of healing. Histological examination of the secretome-treated group showed improved re-epithelialization and better healing capacity. In addition, this group showed an increase in collagen content, the formation of new blood vessels, and the capacity to promote their maturation. Conclusions: Secretome-derived therapy may be a safe and effective way to encourage mucosal repair. It might function as a novel, cell-free treatment strategy. As so, it offers regenerative medicine as a possible substitute for conventional cell therapy.