Skip to main content

Assessment of cattle tick infestation: Molecular insights into Rhipicephalus annulatus and the efficacy of garlic oil and nanoemulsion as acaricidal agents

مؤلف البحث
Salwa Mahmoud Abd-Elrahman, Ahmed Kamal Dyab, Fatma Atea Kamel, Abeer A. Khedr, Mervat M. Khalifa , Shaymaa M. Mohamed, Sara Salah Abdel-Hakeem
تاريخ البحث
قسم البحث
مجلة البحث
Veterinary Parasitology
الناشر
elsevier
عدد البحث
329
موقع البحث
https://www.sciencedirect.com/science/article/pii/S0304401724000992?dgcid=coauthor
سنة البحث
2024
المشارك في البحث
صفحات البحث
110211
ملخص البحث

Ticks, particularly Rhipicephalus annulatus, pose significant threats to livestock, causing economic losses and transmitting various infectious diseases. This study aimed to evaluate the potential acaricidal properties of garlic oil and its nanoemulsion against ticks infesting cattle, Rhipicephalus annulatus through the evaluation of mortality rate and morphological changes of the treated ticks. The study also included prevalence, risk factors, and molecular confirmation of tick species. Genetic characterization confirmed the identity of R. annulatus. Our results revealed a high prevalence of R. annulatus (46.9%) with a higher risk in male cattle (50%) than females (44.9%) and a nonsignificant high infection (49.1%) in animals ≤ 1-year-old. The acaricidal efficiency of garlic oil and its nanoemulsion was concentration and time-dependent. The high concentration of garlic oil (20 mg/L) induced complete mortality within 48 hours. The nanoemulsion formulation enhanced efficacy, particularly at 5 mg/L, which exhibited rapid and substantial acaricidal activity. Scanning electron microscopy revealed morphological alterations induced by garlic oil and its nanoemulsion, including changes to the anterior capitulum, dorsal, and ventral cuticles. The study contributes to the exploration of effective, safe, and eco-friendly alternatives for tick control. Further research is warranted to validate their efficacy under diverse conditions and assess practical strategies.