Antibacterial and wound-healing potential of PLGA/spidroin nanoparticles: a study on earthworms as a human skin model

Faculty of Pharmacy

Assiut University

Antibacterial and wound-healing potential of PLGA/spidroin nanoparticles: a study on earthworms as a human skin model

Research Authors
Fatma El-Zahraa A Abd El-Aziz , Helal F Hetta , Basma N Abdelhamid & Noura H Abd Ellah
Research Date
Research Journal
Nanomedicine
Research Website
https://www.futuremedicine.com/doi/abs/10.2217/nnm-2021-0325
Research Year
2022
Research Member
Noura Hassan Abd Ellah
Basma Nagy Abd El-Hamid GadElrab
Research Abstract

Aim

The essential protein element of spider silk ‘spidroin’ was used to assess its impact on the wound-healing process.

Methods

Spidroin nanoparticles (NPs) were prepared using poly(lactic-co-glycolic acid) polymer (PLGA/spidroin NPs) at different weight ratios (5:1, 10:1, 15:1) and were in vitro characterized. The optimized NPs were tested in vitro for release and antibacterial activity. To assess wound-healing effects, NPs were topically applied on surgically induced injuries in Allolobophora caliginosa earthworms as a robust human skin model.

Results

Optimized NPs (173 ± 3 nm) revealed considerable antibacterial effect against Staphylococcus aureus and Escherichia coli. After 4 days of NPs application on wounds, macroscopical and histological examinations revealed a significant reduction in wound and re-epithelialization times.

Conclusion

PLGA/spidroin NPs may represent a promising option for wound …