Galactosamin plus endotoxin has been used widely as model of hepatitis. But the acute effect of this combination on hepatic and extrahepatic biotransformation has not been determined. Therefore, either 2 or 4 hr after a single i.p. dose of 300 mg/kg galactosamine plus 30 ug/kg LPS was administered. Serum, liver, kidney, intestine and spleen were collected. Serum enzymes (AST, ALT,SDH & GGT) were elevated dramatically 2 or 4 hr after treatment. Cytochrome P450 activity toward benzo[a]pyrene was increased in kidney 4 hr after tratment, wheras dealkylation of 7-methoxycoumarin or 7-ethoxresorufin was unchanged in any tisuue at either time point. An increase in UDP-glucuronsyltransferase activity toward 4-methylumbelliferone and 4-hydroxbiphenyl was noted in the intestine. Conjugation of CDNB with GSH was increased in intestine and spleen after 4 hr treatment. GSH content was increased by different amounts depending on which organs were studied 2 or 4 hr after treatment