Breast cancer is the most common cancer affecting women, and plasma-activated water (PAW) is emerging as a potential therapeutic approach. While early results are encouraging, additional research is necessary to assess its long-term effects and identify the most effective methods of application. Therefore, this study aimed to investigate the effects of PAW on an MCF7 breast cancer mouse model.

A total of eighteen BALB/c mice were divided into three groups (n = 6/group): one negative control group and two tumor groups injected with MCF7 cells. After 91 days of MCF7 injection, the tumor groups received either distilled water (DW) or PAW (for 3 min) twice weekly via oral gavage for 28 days.

The results revealed that the conductivity, H₂O₂, and NO₂- values were significantly higher for PAW than for DW, while pH was lower (P < 0.001). Hematological analysis showed an increase …