Gluathione is an important free radical scavenger and a decline in its tissue level is indicative of increased oxidative stress in diabetes. In this study treatment of rats with STZ induced-IDDM and hepatotoxicity as seen by elevation of plasma AST,ALT &GGT. Also, diabetic rats had a loss in the body weight and increased in liver weight. GSH and GR activity were significantly dcreased while GST and GGT ase activity increased in rats treated with STZ. Moreover, diabetic rats showed increase in the rate of bile flow, while biliary efflux of GSH was decreased. Biliary excretion of lysosomal glycosidases activities with 4-methylumbelliferyl were significantly increased in diabetic rats than normal.