Evaluating the performance of rejuvenated asphalt mixes is crucial for pavement design and construction, as using a rejuvenator not only boosts recycling and contributes to positive effects on the environment but also increases the sensitivity to rutting and moisture. This study was executed to evaluate the effect of a warm mix asphalt (WMA) antistripping agent, namely nano-ZycoTherm, on the moisture-induced damage and rutting potential of asphalt mixtures containing 30% and 60% aged (RAP) binder and rejuvenated with 12%  waste engine oil (WEO). For this purpose, the rutting resistance of asphalt mixes in wet and dry conditions was  examined utilizing a loaded wheel tracker. In addition, the impacts of moisture on the performance of the mixtures were evaluated using different experiments, such as modified Lottman (AASHTO T283), resilient  modulus, dynamic creep, aggregate coating and wheel tracking tests. Fourier transform infrared (FTIR)  spectroscopy and thermogravimetric (TG) analysis were performed to identify the functional groups, which  would be significant in terms of moisture damage, and to assess the thermal stability of binder  samples, respectively. The results revealed that the rejuvenation of aged binder with WEO increases  the moisture susceptibility of the mixtures; however, the addition of ZycoTherm was found to enhance the  moisture resistance of WEO-rejuvenated mixtures. Furthermore, the results indicated that the WEO-rejuvenated  mixtures modified with ZycoTherm exhibited a better rutting resistance in a wet condition compared to that of  WEO-rejuvenated and conventional HMA mixtures. However, the rejuvenated mixtures modified with ZycoTherm  showed poorer rutting performance in a dry condition. In summary, the adoption of the WMA antistripping agent,  RAP binder and WEO rejuvenation techniques demonstrated satisfactory outcomes in terms of rutting resistance  and moisture susceptibility, and also, these techniques are much less expensive to implement.