The use of natural materials in modern times is one of the interests of
science. In recent years, there has been a rapid and exciting development in
the use of biologically- based organic materials, for example, innovative drug
treatments, with a focus on addressing, diabetic wound healing, cancer, and
infectious diseases. Focus on tissue responses, chitosan has been registered as
a representative lightweight organic material for its distinctive properties, such
as formability, processability, non-toxicity, and chemical activity, in addition
to its compatibility with chemical flame and chemical sensitivity. Most
importantly, it has antimicrobial activity and promotes wound healing. In this
work, the applications of chitosan were used as a wound dressing. Optimized
chitosan was estimated for wound healing through topical cream application
for three successive days on surgical cuts made in (Lumbricus castaneus)
earthworms, as an alternative for human skin. The outcomes showed
conspicuous decreases in both wound closure and re-epithelialization duration
in chitosan-treated earthworms as evident through macroscopic examination
and thorough histological scrutiny utilizing light and electron microscopy, in
comparison with the control group. In summation, these findings posit that a
wound dressing in chitosan holds promise as a catalyst for wound healing.
Future endeavors will delve into scrutinizing the intricate mechanisms
underlying this influential phenomenon.