Clothes examination may provide important clues in crime investigation. In addition to its role in identification of the victim and sometimes the assailant. It may carry some physical evidence which have the potential to play a critical role in the overall investigation and resolution of a suspected criminal act. Clothes may be disordered indicating a struggle, or may be soiled or stained with blood or other biological stains including semen, hair, saliva or non biological evidence as fibers, paint, glass, debris and dirt as a result of contact with assailant or the scene. The interposition of clothing may prevent the features of secondary projectile deposition and/ or searing from occurring, and the examination of clothing by forensic scientists may provide essential evidence in cases involving firearms. These evidences shouldn't be missed or overlooked. So clothes should be photographed, examined thoroughly and described carefully before beginning the examination of the body. Clothes shouldn't be removed except by the medical man after recording the condition of rigor. Cuts or injuries sustained by the clothing should be carefully compared with the underlying surface of the body. It should be dried preserved carefully in special bag or envelope and seal with sealing- wax impressed with the private seal of the examiner. Chemical and or serological examination may be required. This paper aimed for recognition of the significance of clothing as a potential source of evidence for forensic scientist, physicians as well as emergency room and intensive care personnel, to adopt and follow the procedure designed to preserve such important evidence.