Nosocomial infections are the most common complications affecting hospitalized patients. The main
purpose of this study was to determinate the frequency of nosocomial microorganisms obtained from
intensive care unit patients admitted throughout 48 h and to detect the most common organisms and
their susceptibility patterns to commercial antimicrobial agents and natural products (essential oils).
Resistance gene was determinate. During 18 months study, 894 bacterial isolates were recovered from
682 clinical samples(urine, blood, sputum, wound) in trauma and chest ICUs of Assuit University
Hospital and trauma ICU of Sohag University Hospital. API 20E test was performed for Klebsiella
pneumoniae isolates (as the commonest organisms). In vitro susceptibility of Klebsiella pneumoniae
isolates to 12 antimicrobial agents Ampicilin; Amikacin, Ciprofloxacin, Tetracycline, Bacitracin,
Amoxclav, Gentamicin, Cefotaxime, Ceftazidime, Imipenem, Meropenem and Chloramphenicol (as
commercial antimicrobial agents) and to Rosmarinus officinalis and Cymbopogen citrates essential oils
was performed using the Kirby-Bauer’s disk diffusion method. PCR Testing for resistance gene for
Klebsiella pneumoniae to carbapenems (imipenem and meropenem). Out of 894 bacterial isolates 210
Klebsiella pneumoniae isolates were detected and confirmed by API 20E. Lowest resistance of Klebsiella
pneumoniae to imipenem and meropenem (1%, 1% in trauma ICU of Assiut university hospital) (17.6%,
11.8%in chest ICU of Assiut university hospital) (3.1%, 4.1% in trauma ICU of Sohag university
hospital). cymbopogen citrates essential oils had the positive effect on carbapenem resistant Klebsiella
pneumoniae isolates rather than Rosmarinus officinalis essential oil. bla CTX-M gene, bla TEM gene
and bla SHV gene were detected as resistance gene for imipenem and meropenem.