Chromatographic fractionation of the alcoholic extract of leaves of Chorisia speciosa A. St. Hil. (Bombacaceae) resulted in the isolation and identification of ten compounds, two of them are firstly reported in the family: β-amyrin (1), and Verbascoside (7), in addition to five compounds firstly isolated from the plant p-hydroxy benzoic acid (3), β-sitosterol-3-O-β-d-glucopyranoside (4), succinic acid (5), astragalin (8) and cinaroside (9), in addition to β-sitosterol (2), tiliroside (6) and rhiofolin (10). Their structures were established by various spectroscopic methods (1H NMR, 13C NMR, HSQC, HMBC and UV spectroscopy) as well as comparison with authentic samples. Biological studies of different extracts of Chorisia speciosa A. St. Hil. showed that the extracts have slightly higher antioxidant activity.