Introduction: Patient safety necessitate the modification for behaviors of health care providers to perceive the wide range of factors of working conditions that affect patient safety and design appropriate of the physical environment to reduce hazards. Aim: Assess nurses perception of factors that affect patient’s safety culture at Assiut University Hospital. Study design: A descriptive correlational. Setting: The study was conducted at Assiut University Hospital. Subjects: the study subject consisted of 301 nursing staff working at Assiut University Hospital. The data collected through self – administered questionnaire which included characteristics of the sample data sheet and patient’s safety culture. Results: There the highest mean scores were among studied nurses in Intensive Care Units as regard to all factors of health working conditions (57.3+9.6) and the highest mean scores were among nursing technical institute as regard to all factors of health working conditions (53.8+12.9).

 Conclusions: There were relation among department, current job, and years of experience with all factors of health working conditions. Recommendations: Training programs should be conducted for nurses about how to ensure patient’s safety in the hospital.