The most important sources of conflict at any educational work-place occurs because opinions, perceptions of personnel are incompatible. The present study aimed to explore effect and levels of conflict among academic teaching staff and their assistant. The study subjects: No. = 102 academic teaching staff. Setting; Faculties of Nursing Assiut and Sohag Universities. Tool of data collection; A structured interview questionnaire consists of two parts; 1) Conflict resolution levels, and 2) Effect of conflict. Results: More than half of academic teaching staff and their assistant Faculty of Nursing Assiut University were average conflict level, and two third of them had positive effect of conflict. Meanwhile the majority of academic teaching staff and their assistant Faculty of Nursing Sohag University their conflict levels need improvement, and conflict affect them negatively. Conclusion; the present study concluded that academic teaching staff and their assistant Faculty of Nursing Assiut University achieve higher percent score than academic teaching staff and their assistant Faculty of Nursing Sohag University as regard levels of conflict resolution Recommendations: based up on study findings it is recommended that, academic teaching staff and their assistant need condensed training about conflict resolutions levels and strategies