Nosocomial respiratory tract infections (NRTI) are the most common nosocomial infections in intensive care units (ICUs).. So the education and awareness among nurses as well as adherence to standard nursing guidelines for prevention of nosocomial respiratory tract infection should be used to reduce frequency of nosocomial infection in ICUs.Aim :the current study was carried out to evaluate the effect of implementing nursing guidelines in reducing (NRTI) in mechanically ventilated patients in ICUs .Methods : The study was conducted at the (ICU) of Minia university hospital. The sample of this study comprised 60 adult patients including both sex, their age ranged from 18-60 years old,. They were selected by convenience randomly assigned two equal groups control (group1) and interventional group (group2) (30 in each). Three tools were used to collect the data in this study ,tool one : Patient assessment sheet,tool two : Preventive nursing guideline ,and tool three: Evaluation sheet .Results and conclusion: The result of the current study revealed that Mean age of the both groups around forty year,and more than fifty percent of the patients in both groups were male. The finding of the current study revealed that, the mean score of body temperature in the study group nearly between normal ranges in all the sevens days of the study.While body temperature of the control group most of patients had hyperthermia in all the days of the study,and There were statistical significance differences between both groups in most of days of the study. there were an improvement of the respiratory status of the study group than control group, and a significant differences was elicited between two group on the most of the days of the study in relation to respiratory assessment .Recommendations: based on the current study finding it can be recommended that hospitals should implement protocols for the prevention of NRTI in mechanically ventilated patients that include each of the practices recommended by the CDC. Training and educational program should be developed to the nursing staff about the standardized care that should be delivered to the mechanically ventilated patients