Abstract Background: Education programs are considered for providing nurses with theoretical and technical information needed to acquire new skills and knowledge. Aim: to assess nurses` knowledge and practice regarding patients who had an endotracheal tube and evaluate the effect of implementing education program on nurses` practice and patient's complications. A Quasi-experimental design. This study was carried out in Trauma and General Intensive Care Unit. The sample of this study consisted of 60 nurses and 120 patients who had endotracheal tube pre/post implementing the program. Tools utilized for data collection were nursing questionnaire sheet, observation checklist sheet, and Patient assessment sheet. The results There were statistical significant differences (P<0.000) in the total nurses knowledge scores regarding endotracheal tube care, pre- implementation was compared with postimplementation and There were statistically significant differences(P<0.000) in total nurses practice scores regarding endotracheal tube care pre- implementation was compared with post- implementation. The patient complication was less oral inflammation absence 70% post- implementing the program. In conclusion: there is the statistically significant difference between the nurse knowledge and practice in pre/post implementing the program. Improving nurses’ knowledge and practice have a favorable effect in preventing post-endotracheal tube care complications.