Background: Nursing leaders need mindfulness, which is an effective intervention for reducing stress, promoting well-being, and enhancing their leadership skills. Purpose: To explore the effect of a mindfulness training program on the well-being and leadership skills of head nurses. Methods: A quasi-study design was used and conducted in a psychiatric and neurological university hospital on a convenience sample of 40 head nurses. Using the cognitive and affective mindfulness scale, the mindful attention awareness scale, the leadership skills questionnaire, and the mindfulness program. Findings: The findings resulted in a significant difference in the effect of the mindfulness program before and after training when applied to leadership skills, especially conceptual and administrative skills. Discussion: Managers must adopt a mindfulness program, integrate it into nursing intervention protocols, and circulate it to all nursing staff because it has a noticeable impact if it is practiced seriously and its results are benefited from.