Background: Girls with hearing disability continue to undervalue the practice of breast self-examination, despite Egypt's ever-expanding programs to raise breast cancer awareness. Aim: To evaluate the effectiveness of the training program for girls with hearing disability about breast self-examination in Assiut City. Research Design: Quantitative research pre/post one group intervention design was applied. Setting: Al-aml school for dumb and deaf girls in Assiut City. Subjects: A convenient sample was used to include 153 adolescent girls with hearing disability. Data collection tools: Two tools were utilized: Tool one: Designed interview questionnaire comprised from three parts: Part (1): Personal data, part (2): Girls’ family history of breast cancer and data about breast self-examination and part (3): Questions to assess knowledge of girls regarding breast cancer and breast self-examination. Tool II: Breast self-examination observational checklist. Results: It noticed from the current results that 46% of girls aged less than 18 years, 90.8% from rural area, 71.9% weren’t know about breast self-examination. The girls’ mean of knowledge and practices regarding breast self-examination was in pre-program 12.28 and 2.26 while it enhanced to be 36.03 and 9.67 with significant improvement after the program application. Conclusion: The training program improved knowledge and practices of breast self-examination among girls with hearing disability. Recommendations: Continuous training program about breast self-examination for girls with special need to increase their awareness regarding early detection of breast cancer.